Lonomia obliqua venom and hemolymph have been shown to contain molecules that, besides interfering in blood coagulation (for a review, Veiga et al. ). Lonomia obliqua (Walker, ) is a moth from the family Saturniidae, widely distributed in tropical rainforests of South America. In its larval stage (caterpillar) it . Rev Assoc Med Bras (). May-Jun;61(3) doi: / Lonomia obliqua Walker (Lepidoptera: Saturniidae): hemostasis .

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In southern Brazil, sinceseveral cases of accidents produced by unwilling contact with the body of poisonous caterpillars of the moth species Lonomia lpnomia Walker, Lepidoptera: In a previous study, it was shown that low concentrations of L.

Lonomia obliqua venom and hemolymph have been shown to contain molecules that, besides interfering in blood coagulation for a review, Veiga et al. The effects of a dose from multiple caterpillars can be dramatic and severe, including massive internal hemorrhagingrenal failureand hemolysis.

This internal bleeding would fill the surrounding tissue with “bruised blood”. The lnoomia study shows that L. The antiapoptotic protein from the hemolymph of L. While this victim did not die, prompt medical attention was necessary.

The antiapoptotic protein alone, at doses of 1. In view of the effects that L. A typical envenomation incident involves a person unknowingly leaning against, placing their hand on, or rubbing their arm against a group of these caterpillars that are gathered on the trunk of a tree.

Cell counting After venom treatment of the UMG cell line, medium was removed, cells were washed in PBS and detached with trypsin 0. Retrieved 15 December The decrease in NO production induced by the venom does not completely explain the proliferative effect of the venom, because, as shown in Fig.


These results suggest that the venom components act at different optimal lonomua, therefore the combined effect may vary depending on sample preparation, concentration used and also cell type. By using this site, you agree to the Terms of Use and Lbliqua Policy. Suitability of human mesenchymal stem cells for gene therapy depends on the expansion medium.

Effects of Lonomia obliqua caterpillar venom upon the proliferation and viability of cell lines

Among animals known to produce pharmacologically active molecules that interfere in human cell physiology, the caterpillar Lonomia obliqua has become the focus of toxicological studies due to recent findings about its venom constituents.

Support Center Support Center. To increase cell yield, many procedures are used, such as supplementing the culture medium with nutrients and mitogenic activators, minimizing the presence of toxic metabolites such as lactate and ammonia, and preventing the lack of oxygen. Fibrinogenolytic and procoagulant activities in the hemorrhagic syndrome caused by Lonomia obliqua caterpillars.

Brazilian Journal of Medical and Biological Research.

[Accidents with caterpillar Lonomia obliqua (Walker, ). An emerging problem].

Lonomia obliqua Lepidoptera, Saturniidae ; 6 th instar larvae. In another case, internal bleeding spread throughout the lower body. We express special thanks to Dr. Lonomia obliqua Walker Caterpillar in Yasuni National ParkEcuador.

Statistical analysis All experiments were performed at least three times. The first step to verify this difference would be to identify the cellular mechanism activated in fibroblasts that lead these cells to death and ogliqua to determine if this same mechanism is activated or not in tumor cells treated with the venom. MTT is a salt that measures the activity of mitochondrial enzymes; once reduced by these enzymes, MTT forms formazan crystals, which can be quantified in a colorimetric assay.


Lonomia obliqua

Culture of mammalian cells represent a major resource for producing high-value bio-therapeutic drugs Wurmwhich includes monoclonal lonmia Rycyzyn obliiqua al. Causes and risk factors for acute dialysis initiation among patients with end-stage kidney disease—a large retrospective observational cohort study. Use dmy dates from May Use American English from November All Wikipedia articles written in American English Articles with ‘species’ microformats All articles with unsourced statements Articles with unsourced statements from May P values less or equal to 0.

Structures involved in production, secretion and injection of the venom produced by the caterpillar Lonomia obliqua Lepidoptera, Saturniidae Toxicon.

Exposure of UMG to crude venom extract led to a decrease in the production of nitric oxide, and activation of the cAMP signaling pathway inhibited the effects of the venom, indicating that these mechanisms may influence cell proliferation triggered by the venom.

Growth inhibition and radiosensitization of cultured glioma cells by nitric oxide generating agents. In vivo effects and molecular aspects associated with the hemorrhagic syndrome”. Please review our privacy policy. Caterpillars of many species can cause irritation by their hollow body hairs that envenom or detach easily, or can be poisonous if ingested; [4] however, prior to investigations into Lonomia caterpillars, it was not known that caterpillars could produce toxins which in sufficient quantities could kill a human being.

Venom samples were prepared from bristles as described in Veiga et al. As anticoagulants have some very beneficial applications e. Published online Jan