Petite H(1), Frei V, Huc A, Herbage D. Author information: (1)Coletica, Lyon, France. Cross-linking of collagen-based biomaterials increases their strength and. By DANIEL HERBAGE, JOSETTE BOUILLET and JEAN-CLAUDE Solubilization of collagen from bovine articular cartilage with pepsin. The formation of new bonds between the denatured collagen chains and the proteoglycans can explain the D. Herbage, A. Huc, D. Chabrand, M.C. Chapuy.
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Both supports were always present during the study and a partial decrease in size and weight was detected only with control sponges, both seeded and unseeded. DPPA-treated pericardium had a resistance to collagenase digestion similar to that of glutaraldehyde- or hydrazine-treated pericardium. In order to validate and quantitate the extent of reaction, the degree of cross-linking was determined by the measure of the free primary amino group content of the samples.
Application of Collagen Scaffold in Tissue Engineering: Showing of 71 extracted citations. Mouse fibroblasts in long-term culture within collagen three-dimensional scaffolds: With the rapid herbagd in tissue engineering, these different biomaterials have been used as three-dimensional scaffolds and cell transplant devices.
Gelatinase A activation is regulated by the organization of the polymerized herbsge cytoskeleton. RosenthalGabriele J Koehler Anticancer research Treatment of native bovine pericardium with 0. Cytological evaluation of capsular tissue surrounding TCPL implant in adult rats.
The Perfect Match for Cardiac Repair? BiomaterialsElsevier,22, pp. These results were corroborated by resistance to bacterial collagenase digestion: Cell proliferation was only noted in the 10 6 cells-seeded sponges 4-fold increase after 4 weeks of culture. Allogeneic Mesenchymal Stem Cells and Biomaterials: These in vitro herbabe indicate that both collagen matrices can support the development of tissue engineered cartilage.
Gelatine-Based Biomaterials and Their Biocompatibility: From This Paper Figures, tables, and topics from this paper.
Collagen-based biomaterials as 3D scaffold for cell cultures: Cross-linking of collagen-based biomaterials increases their strength and persistence in vivo. In the 10 7 cells-seeded sponges, mineral deposition, observed with unseeded sponges, was significantly decreased 2- to 3-fold. Collagen as matrix for neo-organ formation by gene-transfected fibroblasts. BonassarCharles A.
Ham A Benghuzzi Biomedical sciences instrumentation Mineral deposition Glycosaminoglycan deposition Articular cartilage repair Collagen-based biomaterial Collagen synthesis. Daniel Herbage 1 AuthorId: Vacanti Journal of cellular biochemistry. Recently, we described an efficient cross-linking process via the formation of acyl azide groups on methylated carboxyl groups of collagen using hydrazine and nitrous acid referred to here collsgen the hydrazine method.
Collagen – References
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Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.
In this report, we propose a simpler, faster way to prepare acyl azide groups and to cross-link hebage biomaterials, using diphenylphosphorylazide DPPA as a reagent. Glycosaminoglycans accumulated in the matrix, up to 1. Showing of herbafe references. Specific cartilage collagens types II and XI were deposited in the matrix throughout the culture and traces of type I collagen were noticed only in the culture medium after weeks cpllagen 4 weeks in the case of 10 6 and 10 7 cells-seeded sponges, respectively.
Similar decreases in free primary amino group content and increases in thermal stability were obtained for collagen films treated with DPPA, glutaraldehyde, or hydrazine. Development and characterisation of a three-dimensional in vitro hepatic fish model to investigate xenobiotic metabolism Matthew G Baron AliDavid J Mooney Current topics in microbiology and immunology References Publications referenced by this paper.