La propagación de plantas, a través de la embriogénesis somática, Tesis presentada en opción al grado científico de Master en Biotecnología Vegetal. UCLV. Embriogénesis somática en papaya cultivar ‘Maradol Roja’: una alternativa para either to produce a high number of plants (high quality vegetal material to be. La regeneración de plantas de cacao mediante embriogénesis somática se ha El manejo del material vegetal se llevó a cabo en cámara de flujo laminar.

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Manual del cultivo de cacao. Journal of Virological Methods, Other difficulties come from the influence of environmental factors on the resulting plants, which influence on the growth, development and morphogenesis of shoots and plants generated in vitro mainly during the trophic phase.

En este ensayo se evaluaron como explantes dos estados de desarrollo de los embriones primarios, embriones tipo globular y embriones embriogenesls cotiledonar. Se realizaron 3 repeticiones por tipo de explante para un total de explantes.

Variation of plants derived from indirect somatic embryogenesis in cotyledon explants of papaya.

Agricultural and Food Science These results also accounted for the pioneering studies on the photoautotrophic culture conditions in Cuba for this plant species [17]. Acta Horticulturae Indirect somatic embryogenesis and morphohistological analysis in Capsicum chinense. Plant Cell Tissue and Organ Culture Dentro de las vias de propagacion in vitro de plantas, la embriogenesis somatica ofrece posibilidades de obtener volumenes de produccion superiores en un menor periodo de tiempo vsgetal a un costo mas bajo, lo cual la convierte en un metodo potencialmente mas eficiente que la regeneracion via organogenesis.

Por otro lado, Von Arnold et al.

Revista Colombiana de Biotecnología

Effect of activated charcoal on Brassica oleracea microspore culture embryogenesis. Use of secondary somatic embryos promotes genetic fidelity in cryopreservation of cocoa Theobroma cacao L. Overview on perennial cultures. Plant Cell Rep A fin de enfrentar estos retos, sera necesario disponer de nuevos conocimientos derivados del avance cientifico.


Ministerio de Agricultura, Programa para el Desarrollo de la Amazonia. Besides, somatic embryo germination is affected by hyperhydricity and callus formation at the base of the explant, leading to incomplete germination [6], and having the in vitro shoots to be passed to a culture medium for rooting.

A parte de ello se encuentra la variabilidad propia de cada clon discutida anteriormente. Los embriones pasaron por fases que se correspondieron con medios de cultivo diferenciales: This was possible due to the increased ventilation of culture vessels, by capping them with metallic foil paper with two holes.

Another technical difficulty comes from the relative nature of papaya as refractive to tissue culture, with low percentages of in vitro establishment, low multiplication rates and the presence of endogenous bacteria when propagated from cultures under controlled conditions.

Regeneration of somatic embryos and roots from Quinche leaves cultured on media with different macroelements composition.

Plan cacaoetero permitira al pais exportar cacao.

PG treatment also improved the morpho-physiological parameters of the plants obtained. Investigadora y asesora en cultivo in vitro vegetal.

These are novel and promising findings which make more efficient the in vitro regeneration system. We also report the first studies in ejbriogenesis under photoautotrophic conditions during the rooting phase, critical for the in vitro culture of this crop.

A comparison between Theobroma cacao L. Regeneration through somatic embryogenesis of an elite colombian Theobroma cacao L.

Topicos de La Embriogenesis Somatica Vegetal

Somatic embryogenesis from immature peach palm inflorescence explants: Another product to be considered is phloroglucinol PGa phenolic compound obtained by degrading floridcin and a precursor in the synthetic pathway of lignin.

Factors affecting maturation of avocado somatic embryos. It is expected that these results could have a positive impact on the propagation of this commercially relevant crop in Cuba in the short time, also setting guidelines for future research on the genetic regulation during SE in papaya species.


So far, these were the first reports on using these growth regulators for the improvements of papaya in vitro cultivation during the most critical phase rooting [18, 19].

Effect of phloroglucinol on rooting and in vitro acclimatization of papaya Carica papaya L. Tissue culture studies on cocoa Theobroma cacao L.

Plant Science Tissue culture studies with Theobroma cacao.

Somatic embryogenesis from integument perisperm cultures of coffee. Nevertheless, a high number emgriogenesis works report callus formation prior to the generation of the somatic embryos, even in the case of using tissues with embryogenic-predetermined cells zygotic embryosthereby increasing the embriogejesis of genetic variability in the plants obtained [5].

Better rooting procedure to enhance survival of field growth Malaysian Eksotika papaya transformed with 1-aminocyclopropanecarboxylic acid oxidase gene. Developmental pathways of somatic embryogenesis.

Another alternative considers the potential introduction of biologically active substances of national production and available for crop improvement in the in vitro regeneration process of papaya, in order to improve rooting in vitrowhile substituting the import of equivalent foreign products. Un total de 67 plantas fueron usadas para este experimento. The study also provided evidence on the role of auxin transporters at all stages of histological differentiation during the development of somatic embryos.

Las fases de estos protocolos somahica Scientia Horticulturae Photoautotrophic micropropagation-environmental control for promoting photosynthesis.

Topicos de La Embriogenesis Somatica Vegetal : Florio De Real Sunshine :

Direct sowing of Coffea arabica somatic embryos-produced in a bioreactor and regeneration of plants. A supplementation of current research. Photoauto- ,Photohetero-and Photomixotrophic in vitro propagation of papaya Carica papaya L.

Esto es coherente con los resultados obtenidos por Traore et al.