Breve descripción acerca de los cromosomas politénicos de Drosophila melanogaster. View Notes – Laboratorio cromosomas politénicos (1).docx from BIO at Javeriana University. Laboratorio Identificacin de Cromosomas Politnicos de. Una herramienta importante para estos estudios es el etiquetado de los cromosomas politénicos con anticuerpos frente a la enzima, el factor de transcripción.

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The dissection forceps we use were mis-identified: Repita los politneicos 4. Caliente para dispersar a la Triton X y su uso dentro de una hora.

Cromosoma politénico

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If that doesn’t help, please let us know. A squash that is too rapid or hard may also cause the chromosomes to float away. Lugar pesos g en el cubreobjetos de las diapositivas. Click here for the english version. Unable to load video. My thought is that they may be getting flushed out during my transfers.


Cromosoma politénico – Wikipedia, a enciclopedia libre

A subscription to J o VE is required to view this article. Coloque un segundo Cromoxomas en la parte superior y aplanar los cromosomas, colocando el pulgar sobre el lugar donde se coloca el cubreobjetos y presionar con firmeza, evitando cualquier movimiento horizontal del cubreobjetos que se cortelos cromosomas.

Is it the PFA?

If you are seeing few salivary gland chromosome sets you may be losing them when you squash. When you put them on the coverslip and then pick up the coverslip with the slide the chromosomes may float away to the edges of the cover slip if there is too much liquid present. You are right about the Triton. Please sign in or create an account. This may be a dumb question but when you put the tissue in Fixative 1 the Triton will lyse the membrane scorrect? If the problem continues, please let us know and we’ll try to help.

Please check your Internet connection and reload this page. Warm to disperse the Triton X Las modificaciones de este procedimiento se describen en detalle en Johansen et al. For other languages click here. Examinar el portaobjetos una vez que el hielo se ha sublimado lejos de confirmar que el tejido adherido a la diapositiva y no quedarse con el cubreobjetos.


The trick is to leave them in just long enough. Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes.

Cromosomas politénicos |

Skip to content Biology. You must be signed in to post a comment. If you leave the glands in too long they will fall apart.

Try placing them on the coverslip in a smaller drop of liquid and being careful about how you apply the pressure when you squash. That is the volume I prepare each time, usually enough for two or three prep. Please recommend JoVE to your librarian. You will only be able to see the first 20 seconds. An unexpected error occurred.